ABSTRACT
Background: The incidence of Candida tropicalis less susceptible to fl uconazole (FLC) has been reported in many parts of the world. Objectives: The aim of this study was to examine the changes of putative virulence attributes of Candida tropicalis accompanying the development of resistance to FLC in vitro and in vivo. Materials and Methods: A FLC-resistant strain (FLC-R) was obtained after sequential exposure of a clinical isolate FLC-sensitive (FLC-S) to increasing concentrations of the antifungal. The course of infection by both strains was analyzed in BALB/c mice. Analyses of gene expression were performed by real-time polymerase chain reaction PCR. The cell surface hydrophobicity, adhesion and biofi lm formation were also determined. Results: Development of resistance to FLC could be observed after 15 days of subculture in azole-containing medium. Overexpression of MDR1 and ERG11 genes were observed in FLC-R, and this strain exhibited enhanced virulence in mice, as assessed by the mortality rate. All mice challenged with the FLC-R died and FLC-treatment caused earlier death in mice infected with this strain. All animals challenged with FLC-S survived the experiment, regardless of FLC-treatment. Overall, FLC-R derivatives strains were signifi cantly more hydrophobic than FLC-S strains and showed greater adherence and higher capacity to form biofi lm on polystyrene surface. Conclusions: The expression of virulence factors was higher in FLC-R-C. tropicalis and it was enhanced after FLC-exposure. These data alert us to the importance of identifying microorganisms that show resistance to the antifungals to establish an appropriate management of candidiasis therapy.
ABSTRACT
O presente trabalho teve comoobjetivo identificar o conhecimento geral que permeia os traços de liderança desenvolvidos nos currículos de graduação em Enfermagem. Uma breve revisão bibliográfica possibilitou uma reflexão a respeito de como essa característica vem sendoarticulada nas reformas curriculares. Considerando a qualidade da assitência deEnfermagem e a satisfação do trabalho em equipe como o ápice da liderança, os órgãos formadores precisam explicitar e operacionalizar conteúdos e estratégias de ensino-aprendizagem que permitam experiências concretas nos acadêmicos.
Subject(s)
Humans , Nursing , Curriculum , LeadershipABSTRACT
The submucous plexus of the normal small and large intestine of Calomys callosus was studied by NADH and AChE histochemical techniques and by transmission and scanning electron microscopy. The plexus contains (X + SD) 7,488 + 293 neurons/cm2 in the duodenum, 5,611 + 836 in the jejunum, 2,741 + 360 in the ileum, 3,067 + 179 in the cecum, and 3,817 + 256 in the proximal colon. No ganglia or nerve cell bodies were seen in the esophagus, stomach, distal colon or rectum. The neurons are pear-shaped with a round or oval nucleus and the neuronal cell profile areas were larger in the large intestine than in the small intestine. Most of the neurons display intense AChE activity in the cytoplasm. AChE-positive nerve fibers are present in a primary meshwork of large nerve bundles and in a secondary meshwork of finer nerve bundles. At the ultrastructural level, the ganglia are irregular in shape and covered with fibroblast-like cells. The nucleoplasm of the neurons is finely granular with a few condensations of chromatin attached to the nuclear envelope. In the neuropil numerous varicosities filled with vesicles of different size and electron densities are seen. The pre- and post-synaptic membrane thickenings are asymmetric. Characteristic glial cells with oval nuclei and few organelles are numerous. These data provide a detailed description of this submucosal meshwork.